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Leah Gottlieb

Leah Gottlieb

Director

Red Nucleus

Technologies

My Portfolio Highlights

My New Course

Introduction to Python

My New Workbook

Course notes: Introduction to Data Science in Python

My New Course

Introduction to Python

Statistical wizard, casting spells of significance on data experiments.

My Work

Take a look at my latest work.

course

Introduction to Python

course

Intermediate Python

DataLab

Course notes: Introduction to Data Science in Python

course

Introduction to Python

DataCamp Course Completion

Take a look at all the courses I’ve completed on DataCamp.

Courses

Introduction to Python
Intermediate Python
Data Manipulation with pandas
Introduction to Statistics

My Work Experience

Where I've interned and worked during my career.

The Janssen Pharmaceutical Companies of Johnson & Johnson | Sep 2021 - Present

Scientist

Biochemistry and Biophysics, DTMP Enabling platforms - Serve as ASMS (Affinity-Selection MS) platform lead on project teams - Develop and implement ASMS analysis of targets to support small molecule drug discovery as well as target engagement studies for a variety of programs across the portfolio - Standardize ASMS workflow, including experimental execution and follow-up as well as data acquisition, storage, data analysis, and reporting. - Uphold industrial safety standards - Rigorously maintain electronic notebook records - Provide ad hoc support for a variety of programs at different stages of the drug discovery pipeline, including target deconvolution and target engagement studies - Communicate progress and findings to stakeholders, ensuring that deliverables are provided within the expected timeframe
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Johnson and Johnson | Sep 2021 - Present

Scientist, Biochemistry and Biophysics

- Serve as ASMS (Affinity-Selection MS) platform lead in project teams - Develop and implement ASMS analysis of targets to support small molecule drug discovery as well as target engagement studies for a variety of programs across the portfolio - Standardize ASMS workflow, including experimental execution and follow-up as well as data acquisition, storage, data analysis, and reporting - Contribute to intact MS workflows - Uphold industrial safety standards - Rigorously maintain electronic notebook records - Provide ad hoc support for a variety of programs at different stages of the drug discovery pipeline, including target deconvolution and target engagement studies - Communicate progress and findings to stakeholders, ensuring that deliverables are provided within the expected timeframe

WIT Legal, LLC | Feb 2021 - Aug 2021

Consultant

N/A

University of Pennsylvania | Sep 2019 - Aug 2021

Postdoctoral Researcher

Advisor: Dr. Ian A. Blair My work focused on using mass spectrometry to monitor and evaluate Frataxin Isoform E involvement in the fatal recessive neurodegenerative disease, Friedrich's ataxia. Some of these studies will include development of quantitative methodologies towards monitoring disease progression in patients. Accomplishments include: - Development of an Isoform E-specific immunoprecipitation assay from blood - Creation of a targeted triple quadrupole mass spectrometry-based method for high-throughput quantification of Isoform E using an internal standard produced in-house - Extensive collaboration with industry partners

WIT Legal, LLC | Aug 2020 - Aug 2020

Expert Witness

additional details.

University of Pennsylvania | Feb 2019 - Aug 2019

Postdoctoral Researcher

- Advisor: Dr. Ronen Marmorstein - Mentored a high school student during the summer session. - Presented work at departmental poster sessions. - Presented work at and participated in departmental structural biology weekly meetings. - Project Description: The conserved N-terminal acetyltransferases (NATs) catalyze the co- translational N-terminal acetylation of the majority of the proteome. Many NATs require regulatory subunits for both robust activity and substrate selectivity. This project aims to use enzymological and structural techniques to probe the molecular roles of the small regulatory subunits, HYPK and Naa38p, in NatA and NatC activity, respectively. NatA and NatC are particularly interesting as they are the only NATs that require a small regulatory subunit for efficient enzymatic activity. Furthermore, the Huntingtin (Htt) yeast two-hybrid protein K (HYPK) has important implications for Huntington’s disease: HYPK overexpression as well as NatA knockdown have both led to the decrease of Htt aggregation associated with disease pathogenesis. Recently, we published our work characterizing the NatA-HYPK interaction. The work done during this short time served as an extension of that work - elucidating the basis of Naa10-type missense mutations and their effects on the NatA complex (published) and studying the NatC complex.

University of Pennsylvania | May 2014 - Jan 2019

Graduate Research Assistant

- Defended Thesis on January 22, 2019 - Advisor: Dr. Ronen Marmorstein - Wrote and submitted F31 grants for review and resubmission. Scored within the top 20th percentile. - Mentored three students over the course of four years. - Presented work at departmental as well as national poster sessions. - Presented work at and participated in departmental structural biology weekly meetings. - Determined two atomic-resolution crystal structures of human enzymes (PDB: 6C95 and 6C9M). - Determined the mechanism of and quantified HYPK-mediated NatA inhibition using enzymatic assays and structure-function studies. - Characterized the biophysical properties of the NatA and NatA/HYPK complexes using calorimetric techniques. - Project Description: The conserved N-terminal acetyltransferases (NATs) catalyze the co- translational N-terminal acetylation of the majority of the proteome. Many NATs require regulatory subunits for both robust activity and substrate selectivity. This project aims to use enzymological and structural techniques to probe the molecular roles of the small regulatory subunits, HYPK and Naa38p, in NatA and NatC activity, respectively. NatA and NatC are particularly interesting as they are the only NATs that require a small regulatory subunit for efficient enzymatic activity. Furthermore, the Huntingtin (Htt) yeast two-hybrid protein K (HYPK) has important implications for Huntington’s disease: HYPK overexpression as well as NatA knockdown have both led to the decrease of Htt aggregation associated with disease pathogenesis. Therefore, we are also interested in investigating the link between HYPK/NatA activity and Huntington’s disease.

University of Pennsylvania | Sep 2013 - May 2014

Organic Chemistry Lab Teaching Assistant

- Managed a laboratory consisting of 12-14 undergraduate students. - Demonstrated and oversaw safe laboratory techniques. - Developed grading rubrics and graded laboratory reports as well as graded laboratory exams.

University of Pennsylvania | Aug 2013 - Dec 2013

Graduate Student Researcher

- Participated in 7-week-long rotations. - Presented and composed summary report of the work completed during rotation period. - Met with student and faculty (listed below) mentors in order to ensure progress. Rotations: Dr. E. James Petersson - Expressed and purified p-Azidophenylalanine mono-substituted alpha- synuclein, which was subjected to strain-promoted click chemistry and maleimide chemistry, in order to perform intramolecular FRET studies. Dr. Ivan Dmochowski - Performed four synthetic steps to produce cryptophane for use as a potential cancer biosensor.

University of Pennsylvania | Jun 2013 - Aug 2013

Visiting Research Scholar

- Mentored by Dr. Tobias Baumgart. - Expressed, purified, and performed maleimide labeling of the peripheral membrane protein, FBP17. - Gained experience with confocal microscopy, electron microscopy, GUV and LUV binding assays, etc.

Binghamton University | Aug 2012 - May 2013

Teaching Assistant

- Maintained a laboratory section consisting of 22 undergraduate students. - Demonstrated assigned experiment as well as safe techniques necessary for experiment completion, and oversaw student safety practices throughout the laboratory period. - Graded laboratory reports as well as material associated with discussion sections. - Participated in weekly meetings with the course director, Alexsa Silva.

Binghamton University | Feb 2012 - May 2013

Tutor

- Tutored in organic chemistry, general chemistry, and German. - Developed weekly lesson plans and assignments to strengthen students' abilities in desired subject areas.

YWCA USA | Sep 2011 - May 2013

Front Desk Receptionist

- Administered security checks and managed residential visitors, which consisted of women who had recently lost access to a stable residence, been released from incarceration, drug rehab programs, etc. - Monitored residents to ensure adherence to tenant agreement and reported disturbances as well as pertinent needs to the available residential assistant and/or the on-call case manager.

Binghamton University | Jul 2011 - May 2013

Undergraduate Student Researcher

- Mentored by Dr. Zhitao Li. - Utilized flash column chromatography and NMR in order to purify and synthesize a restrained chair mannose sugar in order to study the stereoselectivity of its glycosylation. Summary: The purpose of this work was to study the stereoselectivity of a mannose sugar in a restrained chair conformation, while the project mainly focused on the synthetic development of this restrained mannose monomer. We hypothesized that glycosylation would yield a ß-anomeric linkage, thus showing that torsional strain influences the formation of the ß-linkage.

Duquesne University | May 2012 - Jul 2012

Student Researcher (NIH-SRE)

- Mentored by Dr. Michael Cascio. - Purified Sf9-produced wild-type Glycine receptor and performed copper- promoted click chemistry coupled with chemical cross-linking in order to develop a structure-function analysis of receptor.

Binghamton University | Aug 2008 - May 2010

ResCon

- Employed troubleshooting and fixed software-related computer problems while instructing clients on preventing future problems with their computers.

Binghamton University | Sep 2007 - May 2008

Newing College Website Manager

- Created, maintained and expanded the Newing College website. - Developed graphics and content for the website. Communicated frequently with RDs, RAs, Hall Presidents, and bosses.

My Education

Take a look at my formal education

Doctor of Philosophy (Ph.D.), Chemistry, Biological DivisionUniversity of Pennsylvania | 2019
Bachelors of Science, Chemistry, German, LinguisticsBinghamton University | 2012
Non-Degree Seeking, Chemistry, German, Spanish, RussianLeipzig University | 2011
High School Syosset Senior High school | 2007

About Me

Leah Gottlieb

Experienced with small molecule early discovery research and high-throughput screening (HTS) using mass spectrometry (HTMS), biophysics, and biochemistry. Current focus areas include improving the HTMS platform workflow using data science.

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