Student of the Data Science Bootcamp online Batch #1181. 9-week full-time intensive coding bootcamp to learn Data Science with Python: Data Analytics with SQL, BigQuery, Pandas, Numpy, and Matplotlib; Statistics with Scipy, Seaborn and Statsmodels;Machine Learning with Scikit-learn; Deep Learning with TensorFlow Keras; and develop Data Products with Google Cloud Platform, ML Flow and Streamlit. Final course project developed CompVis - an application using Deep learning for face recognition to use in Industrial Safety, based on TensorFlow transfer learning, FastAPI and a Streamlit app frontend: https://compvis.streamlit.app/ My GitHub profile: https://github.com/dosorio79

i3S - Instituto de Investigação e Inovação em Saúde | Jan 2022 - Oct 2022

Project leader, post-doctoral researcher

Secured Portuguese Government funding (50.000 €) in a competitive national project application. Studied the roles of different actin crosslinkers in sarcomere connectivity geared towards the use of C. elegans as a muscle disease model

IBMC.INEB Associate Laboratory | Sep 2013 - Dec 2021

Postdoctoral Researcher

Working in Ana Carvalho's lab studying the role of non-muscle myosin II in cytokinesis in C. elegans.

Universty P.Curie UPMC-Paris6 | Jan 2013 - Sep 2013

Post-doc

Following up on results from my Doctoral thesis, in particular the involvement of nuclear envelope proteins in nuclear movement

INSERM | May 2007 - Jan 2013

PhD thesis in Biomedicine

In 2007, for my PhD thesis in Biomedicine, I joined the newly created lab of Dr. Edgar Gomes at the Pierre et Marie Curie University (Paris). Migrating fibroblasts on a wound edge polarize by moving their nucleus rearwards and this movement depends on retrograde actin flow coupled to the SUN2- Nesprin2 complex (LINC complex) at the nuclear envelope. To identify novel proteins involved in this process, I established a dataset of 230 potential nuclear envelope proteins based on the data from published mass- spectrometry studies and performed a live-imaging siRNA screen for nuclear movement. I identified 26 hits inhibiting nuclear movement and was involved in the biological characterization of two. Samp1 is a protein of the inner nuclear membrane and we’ve shown that it colocalizes and co-migrates with the LINC complex during nuclear movement. Furthermore, we found physical interactions with SUN2 and Lamin A/C, indicating that Samp1 stabilizes the LINC complex to the nuclear lamina for effective nuclear migration. This work was published J Cell Sci. 2012 Mar 1;125(Pt 5):1099-105 (IF 6.401). Ndc1 is a nuclear pore protein and I found it also interacts with the LINC complex, in particular the SUN2 protein. Rescue experiments and localization analysis suggest a role independent of the nuclear pore complex, thus potentially uncovering a novel function for Ndc1. This work is in advanced stage of preparation for publication.

Marine Biological Laboratory | Jun 2009 - Jul 2009

Phisiology Course '09

Selected for the 2009 edition of this prestigious course. The MBL Physiology Course, founded by Jacques Loeb and one of the oldest continually running biology courses in the world, has a distinguished history that includes the discovery of cyclin B, which led to a Nobel prize for Tim Hunt or the discovery of Kinesin by Lasker awardee Ron Valle. The Course traditionally has had three goals: graduate training, cutting edge research, and introducing new generations of scientists to the unique environment of the MBL. There is a particular emphasis in quantitative analysis and computational modeling of cell physiology. This direction is necessary to take the parts lists of cellular processes, and turn them into true understanding.

Orphanet-Portugal | Jan 2003 - Apr 2007

Information manager

As information manager of the European Network of Rare Diseases - Orphanet for Portugal I was in charge of the communication and editorial tasks of the project including: translating disease summaries from English to Portuguese; performing contacts with clinics, labs, patient associations and registries pertaining rare diseases; keeping the national website updated; performing outreach presentations at congresses, universities and government bodies. During this time I interacted with an extensive European network of partners, in particular the project leaders in Paris France (INSERM SC11)

Universidade do Porto | Jan 2005 - Jan 2007

MSc thesis in Molecular Genetics

and Computational Chemistry laboratory, Faculty of Science, Porto, Portugal under the supervision of Prof. Maria João Ramos and Dr. Agostinho Antunes. I studied the molecular evolution of the angiogenin gene in primates, a peculiar enzyme of the RNase 5 family that has a reduced RNase activity but promotes angiogenesis in normal and pathological situations. I found 15 sites of the gene were under positive selection (accumulating non-synonymous substitutions), most of which corresponded to drastic changes in amino acid properties. By structural modeling I realized that these sites clustered on the main functional regions implicated in angiogenesis that conversely were conserved in other proteins of the RNase A family, indicating an evolutionary pressure on the angiogenin gene to develop its divergent functions. This work was published in BMC Evol Biol. 2007 Sep 20;7:167 8 (IF: 3.52).

Utrecht University | Mar 2002 - Aug 2002

Undergrad Student

In 2002 as an Erasmus student I joined the lab of Prof. Karel Wirtz in the Institute of Biomembranes, Utrecht University for a period of six month. The lab was interested in studying the effects of reactive oxygen species in the vascular endothelium. During my stay I used an in-house developed oxidation sensitive probe (AcetylTyrFluo) and two-dimensional electrophoresis to identify proteins that were oxidized in human endothelial cells upon hydrogen peroxide treatment. Although I left the project before I reached the stage of protein identification by mass-spec, it allowed a great training in proteomics.

IBMC.INEB Associate Laboratory | Mar 2001 - Sep 2001

Trainee-Undergrad

In 2001 I volunteered as an intern in the Amyloid unit (currently Molecular Neurobiology) of the IBMC (Porto, Portugal) under the supervision of Prof. Rosario Almeida. The project focused on the discovery of compounds that could prevent the formation of transthyretin amyloid fibers, a hallmark of paramyloidosis. I performed cloning and expression of protein mutants used for in-vitro testing of the anti-amyloidogenic properties of natural compounds.

My Education

Take a look at my formal education

Data Science, Computer ScienceLe Wagon | 2023

Research Doctorate, Biomedical Sciences, GeneralUniversidade do Porto | 2013

MSc, Molecular GeneticsUniversidade do Minho | 2007

BSc, BiochemistryUniversidade do Porto | 2003

About Me

Data Scientist with a background as a scientific researcher seeking roles to leverage my analytical thinking, problem-solving, and teamwork skills, to make significant contributions and drive innovation in your company.

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