Beta

Laurie Lambot

Postdoctoral position

University of Chicago

Technologies

DataCamp Course Completion

Take a look at all the courses I’ve completed on DataCamp.

My Work Experience

Where I've interned and worked during my career.

University of Chicago | Sep 2018 - Present

Postdoctoral Scholar

Challenged the currently widespread simplistic view of plasticity in the neocortex as exclusively relying on changes in synaptic weight, and demonstrated that neuronal engram integration relies on use-dependent changes in membrane excitability. • Independently applied hands-on scientific experience and knowledge to assess neural activity by using: mouse lines with specific deficits in plasticity pathways, complemented by an innovative mix of imaging (in vivo 2-photon microscopy, optical intrinsic imaging,suite-2p), machine learning-based behavioral tracking (DeepLabCut, Google Colab, Jupyter Notebook) and sophisticated analytical approaches (statistical model-based encoding techniques, MATLAB). • Provided leadership and supervision for direct reports of undergraduate students within or across research groups: developing objectives, monitoring progress, mentoring and teaching new procedures, and providing performance feedback. • Edited and proofread articles, grants, abstracts, posters, oral and visual presentations for style and content. •Performed laboratory protocol management: developing new surgical protocols, writing amendments, conducting the required facility inspections, performing audit surgical procedures for the IACUC and biosafety offices. • Created innovative custom-made tools,mouse behavioral apparatus, and imaging environments by robotics and 3-D digital fabrication methods (laser cutting, 3-D printing, CAD/CAM). • Drove mouse colony management, planning of mouse breeding schemes, and developing genotyping strategies for the establishment of new transgenic lines. • Wrote, assisted, and reviewed grant proposals for research funding (NIH). Keywords: somatosensory cortex, Chronic cranial window surgery, viral vector, neuronal population dynamic, intrinsic plasticity, long-term memory. Principal Investigators: Jason Maclean and Christian Hansel.

Northwestern University - The Feinberg School of Medicine | Feb 2016 - Jun 2018

Postdoctoral Researcher

Investigated the input and output circuits of corticospinal neurons from forelimb-related areas of motor cortex. • Implemented highly efficient, less invasive spinal cord surgery. • Designed, cloned and tested plasmids for custom AAV production. • Implemented strategy to express optogenetic tools in subpopulation of cortical projecting neurons via intersectional AAV injections. • Characterized neuronal connectivity/morphology via confocal/two photon microscopy, clarity tissue clearing technique, and 3D reconstruction (Imaris). • Validated drugs by local application during patch clamp recording on acute brain slices. • Managed pre-review and amendments of IACUC and biosafety protocols. • Participated in the general management of the laboratory: Accomplished pre-review and amendments of IACUC and biosafety protocols, managed maintenance and purchase of new equipment, ensured laboratory safety regulations were met, etc. Keywords: Sensori-motor cortex, stereotaxic brain injection, spinal cord surgery, synaptic connectivity, optogenetic tool, chemogenetic tool, brain mapping. PI: Gordon Shepherd.

IRIBHM - Pierre Vanderhaeghen's Lab | Jan 2015 - Jan 2016

Visiting Research Scientist

Spearheaded electrophysiology and imaging in a collaborative neurophysiology research project with Johnson & Johnson (Janssen pharmaceutica NV, Stellar collaborative project) to develop a molecular toolbox to study human neuronal function and connectivity in health and neurodegeneration using embryonic and induced pluripotent human stem cells. • Implemented protocols to extract iPSC/ESC-derived neurons transplanted mice for cell sorting experiments. • Monitored synaptic formation and neuronal activity in human neurons by single cell electrophysiological recording and imaging of brain slices from iPSC/ESC-derived neurons transplanted mice. • Supervised graduate student in the subject. • Trained graduate student for patch-clamp experiments and imaging. • Presented finding to Janssen and European leaders in the field. • Supported for four month post graduate fellowship to ensure continuity of electrophysiological and imaging data collection. Keywords: FACS, electrophysiology, confocal imaging, human IPSC. Advisor: Pierre Vanderhaeghen

Université libre de Bruxelles | Jun 2010 - Aug 2015

PHD Fellow

N/A

Collège de France | Jul 2014 - Jul 2014

Visiting PHD Student

• Investigate and researched protocols to induce long-term protentiation of striatal medium spiny neurons. Advisor: Laurent Venance

Athénée Royal de Jambes | Mar 2010 - Apr 2010

High School Science Teacher

specialized education. • Developed contextually relevant curriculum to match core science standards building on students core interests.

Université catholique de Louvain | Jan 2007 - Jan 2009

Visiting Research Scholar

Worked with four labs. During the lab rotations, I engaged with peers and senior faculty in cellular and molecular biology work. • Project: Strucural and functional study of PEX1 and PEX6, proteins involved in glycosoma biogenesis in Trypanosoma brucei. Advisor: Fred Opperdoes Research unit of tropical disease (TROP) – Christian de Duve institute (DDUV) • Project: Study of the AMPK/insulin effect on the AS160 phosphorylation and glucose uptake. Advisor: Luc Bertrand Pole of cardiovascular research (CARD) – Institute of experimental and clinical Research • Project: Study of the effects of interleukin 9 at cellular and molecular level. Advisor: Jean-Christophe Renaud Experimental medicine unit (MEXP) – Ludwig Institute for Cancer Research • Project: Study of the Src influence on cellular morphology, proliferation and guidance. Advisor: Pierre Courtoy Cell Biology Unit (CELL) – Christian de Duve institute (DDUV) Keywords: Molecular biology, cellular biology, cell culture, imaging.

My Education

Take a look at my formal education

Python Developer Zero To Mastery Academy | 2022

Doctor of Philosophy - PhD, NeuroscienceUniversité libre de Bruxelles | 2015

Master's degree, Biomedical Sciences, GeneralUniversité catholique de Louvain | 2009

Bachelor's degree, Biomedical Sciences, GeneralUniversité catholique de Louvain | 2007

Northwestern University - The Feinberg School of Medicine | Invalid Date

About Me

Neuroscientist

Create Your Data Portfolio for Free