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Marcus Mahar

Marcus Mahar

Bioinformatics Scientist II

Ambry Genetics

Technologies

My Portfolio Highlights

My New Track

R Programmer

My New Course

Introduction to Python

My New Track

R Programmer

Quantitative navigator, guiding decision-makers through the sea of data.

My Work

Take a look at my latest work.

course

Introduction to Python

course

Intermediate Python

course

Introduction to Python

DataCamp Course Completion

Take a look at all the courses I’ve completed on DataCamp.

Courses

Introduction to Python
Intermediate Python
Data Manipulation with pandas
Supervised Learning with scikit-learn

My Work Experience

Where I've interned and worked during my career.

Ambry Genetics | Mar 2023 - Present

Bioinformatics Scientist II

N/A

Guardant Health | Apr 2022 - Jan 2023

Bioinformatics Scientist II

Lead BI Scientist for exosome RNA-seq pipeline development - Languages: Python | R | Unix | SQL - BI tools: bcl2fastq | STAR | bowtie | samtools | bedtools | DESeq2 | EdgeR - Infrastructure: SGE/HPC | Snakemake | SQL
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Invitae | Sep 2021 - Apr 2022

Bioinformatics Data Scientist - Technology Development

BI Scientist responsible for ad-hoc analysis related to product development and automation of IVD and QC data analysis. - Languages: Python | R | Unix | SQL - BI Tools: fastQC | samtools | bedtools | IGV | bowtie - Infrastructure: SQL | AWS | Docker | Tableau

Invitae | Sep 2020 - Aug 2021

Scientist II - Technology Development

ArcherDX is now Invitae Licenses Differentiated Cells to Return to the Cell Cycle Bioinformatics: ------------------- - Performed and reported on biostatistical analysis in support of product development - Developed and maintained data aggregation, visualization, and reporting infrastructure Languages: Python | R | SQL | Unix Infrastructure: AWS | Docker | LIMS General: ---------- - Authored and reviewed protocols/reports in support of product development and V&V.

ArcherDX, Inc. | Sep 2019 - Aug 2020

Scientist I - IVD Assay Development

Bioinformatics: ----------------- - Designed and wrote analytical pipelines to compile patient sample screening data for use in verification and validation studies, research and development, and bioinformatics software improvement. Languages: R | SQL Infrastructure: AWS Wet Lab: ------------ - Prepared hundreds of RNA, DNA, and ctDNA libraries in support of V&V studies General: ---------- - Conducted NGS-based experiments, performed Root Cause Analysis, and authored/reviewed protocols and reports in support of regulatory submissions to the FDA. - Worked with cross-functional teams, including R&D, Quality Control, Quality Assurance, and Software, to resolve various project-related issues.

Washington University School of Medicine in St. Louis | Aug 2013 - Sep 2019

Doctoral Candidate in Developmental, Regenerative, and Stem Cell Biology

Thesis Lab: Dr. Valeria Cavalli, Department of Neuroscience. Focus: Utilization of RNA sequencing and phenotypic screening to identify new molecular and pharmacological targets for promoting central nervous system axon regeneration in response to traumatic brain and spinal cord injury. Bioinformatics: ------------------ - Developed an RNA-seq pipeline to identify high confidence molecular regulators of axon regeneration. Languages: R | Unix BI Tools: STAR | Bowtie | samtools | bedtools | subread | htseq | DESeq2 | EdgeR | MEME suite Infrastructure: HPC Wet lab: ----------- - Developed several medium-to-high throughput in vitro assays to enable efficient verification of molecular or pharmacological targets to promote axon regeneration - Used adeno-associated virus (AAV) for in vivo validation of novel targets in various models of traumatic nerve injury - Molecular Biology: Cloning, Library Preparation, qPCR, lentivirus production, immunofluorescence - Cell Culture: Dorsal root ganglia neurons, Cortical neurons, 293T cells, transient transfection, generation of stable cell lines, and development of high throughput phenotypic assays. - Animal Models: Mouse colony maintenance, genotyping, intrathecal injection of AAV8, sciatic nerve injury, spinal cord injury, optic nerve injury, and extensive post-operative animal care. - Microscopy: Episcopic and brightfield microscopy on both Nikon and Biotek platforms - Biochemistry: Western blotting Leadership/Communication: --------------------------------- - Utilized strong scientific writing skills and breadth of scientific knowledge to secure >$80,000 in project funding. - Supervisor for one technician, five rotation students, and one undergraduate researcher.

North Dakota State University | Jan 2012 - Jul 2013

Teaching Assistant

-Teaching assistant for three courses: Foundations of Sciences (Univ. 150, Spring 2012), Introduction to Neuroscience (Psych. 260, Summer Session I), and Introduction to Neuroscience (Psych. 260, Summer Session II).

North Dakota State University | Aug 2010 - Jul 2013

Undergraduate Researcher

-Advisor: Dr. Mark Nawrot, Chair of Department of Psychology

North Dakota State University | May 2011 - Sep 2012

Assistant Lab Manager and Undergraduate Researcher

-Advisor: Dr. Linda Langley

My Education

Take a look at my formal education

Doctor of Philosophy - PhD, Developmental, Regenerative, and Stem Cell BiologyWashington University School of Medicine in St. Louis | 2019
Bachelor of Science - BS, PsychologyNorth Dakota State University | 2013

About Me

Marcus Mahar

I'm a former wet lab scientist turned data scientist/evangelist. I use my unique skillset to help wet lab scientists maximally contribute to R&D efforts.

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