Non-heme iron dioxygenases are versatile enzymes in biology and found in all life forms, including the human body. They catalyse a large variety of chemical reactions, including the biosynthesis of natural products, e.g. hydroxyproline in mammals. However, They are also involved in the biodegradation of toxic compounds, for example, toxic cysteine in the brain. In many of these reaction pathways, a regioor stereoselective reaction occurs, which makes this enzyme class of interest to the biotechnology industry for the production of high-value compounds. Many questions remain about the high selectivity patterns of non-heme iron enzymes and how the enzyme drives these mechanisms. Therefore, a detailed computational study was performed on a set of well- characterised non-heme iron dioxygenases with different functions in Nature. In particular, this dissertation focuses on three non-heme iron dioxygenases, of which a recent crystal structure was determined. These structures have been used to elucidate the reaction mechanism and the origins of the selectivity patterns. The works described in this thesis show that the second-coordination sphere of the enzyme plays a vital part in substrate binding and positioning. Moreover, electrostatic and polar interactions from the second coordination sphere often induce a local dipole moment or electric field effect and drive the reaction to an otherwise thermodynamically unfavourable pathway. My work has given insight into the intricate details of enzyme design and how regio- and chemoselectivities can be achieved. This insight can be used to engineer non- heme iron dioxygenases and give them original reactivity patterns with high selectivity for biotechnological applications. Our work has given insight into the mechanism of the herbicide biodegradation and the substrate scope of the enzyme. My work highlight the second coordination sphere groups that induce a local electric field and guide the reaction to the wanted products.

The University of Manchester

Supervisor to intern/ MSc/ MEng students for their dissertation research projects

[Research Asistant] 1. April.2018-31.May.2022 [Project #1- PhD 1st -year] MSc Dissertation, May- September 2018 ▪ Supervision of two MSc students: Nasser Ahmad (Kuwait) and Awad Alenezi (Saudi Arabia), and one MEnge student: Ze Qing Lim (Malaysia), to do the research, which resulted in more efficiency in writing the dissertations, teaching them how to try and error different methods to conduct the simulation of enzymatic mechanism research successfully. [Project #2- PhD 2nd -year] MEnge Dissertation, September 2019- May 2020 ▪ Supervision of one MEnge student: Ze Qing Lim (Malaysia), to do the research, which resulted in more efficiency in writing the dissertations, teaching them how to try and error different methods to conduct the simulation of enzymatic mechanism research successfully [Project #3- PhD 3rd -year] MSc Dissertation, May- September 2020 ▪ Supervision of two MSc students: Ahmed Al Dhurais (Saudi Arabia) and Taha Zaweti (Libya), to do the research, which resulted in more efficiency in writing the dissertations, coaching them on how to conduct the simulation and elaboration on the computational theory. [Project #4- PhD 4rd -year] MEng Dissertation, February- May 2021 & June- September 2022 ▪ Supervision of two MEng students: Aakash Nagwekar (UK), Annemarie Briggs (UK), and One MSc student: Liu He (China), to do the research, which resulted in more efficiency in writing the dissertations, coaching them how to conduct the simulation and demonstrate on the computational theory.

The University of Manchester

The University of Manchester- Teaching Assistant

[Teaching Assistant] Demonstration and Tutorial to undergraduates and MSc students and give constructive feedback • Led tutorial and online tutorial to undergraduate, MEng, MSc students; Initiated topics including Molecular Modelling & Simulation in Chemical Engineering, Batch Processing, Process Engineering Fundamentals, Engineering Mathematics, Oil and Gas Processing, and Engineering Biotechnology. • Read student's reports; Created a system to evaluate papers and give scores.

The University of Manchester

The University of Manchester- Lab Demonstrator

[Lab Demonstrator] [Demonstrate to 1st & 2nd-year undergraduates in Chemical Engineering Lab] [semester 1, 2 (17th September 2018 – currently 2021)] Laboratory Projects 1&2 2018-20 Full Year • Lab Demonstration to the 1 st & 2 nd year of undergraduates, i.g. one semester, demonstrate the experiment, and marking their report, interview them individully, for 12 different students per week and last for 8 weeks, which in total 96 undergraduates, plus online Zoom demonstrate during this pandemic. • The topic ranges from Liquid Liquid Extraction, Transient heat transfer, Fluid Friction in Pipes, Fluid flow in fittings and pipe bends, Wilson Plot Heat Transfer, Fluidised Bed Heat Transfer, Volumetric Flow and Valve Characteristics, and Material and Energy Balance. • Assessment type including Interviews, Lab reports, and Posters.

Manchester Institute of Biotechnology

The University of Manchester/ Manchester Institute of Biotechnology- Internship

Using Infrared and Raman spectroscopy and infrared spectrum pre-processing Principal component Analysis (PCA) algorithm, and Resonant Mie Scattering algorithm (RMieS EMSC) for biochemical sample using Matlab software to differentiate and investigate a range of different bladder cells to acquire the biological data using FTIR on a glass as the substrate in the high wavenumber region is possible.

My Education

Take a look at my formal education

PhD student, BiotechnologyThe University of Manchester | 2022

理科碩士, Advanced Chemical Engineering英國曼徹斯特大學 | 2017

大同大學

About Me

As an experienced researcher, a highly dedicated and naturally conscientious PhD researcher with a portfolio of Chemical Engineering background (STEM domain) over 9 years in academia and 2 years of start-up business experience.

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