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Pamela Craven

Senior Scientist

Pfizer

Technologies

My Portfolio Highlights

My New Course

Introduction to Python

My New Project

The GitHub History of the Scala Language

My New Track

Data Scientist

My New Course

Introduction to Python

Quantitative guru, enlightening the world with data-driven wisdom.

My Work

Take a look at my latest work.

course

Data Manipulation with pandas

course

Introduction to Python

course

Intermediate Python

course

Data Manipulation with pandas

DataCamp Course Completion

Take a look at all the courses I’ve completed on DataCamp.

Courses

Introduction to Python
Introduction to SQL
Introduction to R
Intermediate SQL

My Work Experience

Where I've interned and worked during my career.

Pfizer | Oct 2017 - Present

Senior Scientist

Development and testing of impurity assays within the Analytical Research and Development Bioassay and Impurity group. Performance of pre-validation, validation, and qualification tests using ELISA, qPCR and ddPCR methods. Authoring of validation protocols, method qualifications, test methods, and analytical reports.
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Sanova Bioscience | May 2017 - Sep 2017

Associate Scientist

Analytical development and testing of Sanova products, building the research analytical laboratory, process development, and collaborating with the team on current and future Sanova products.

LFB Biomanufacturing | Oct 2015 - May 2017

Research Associate II

Development of ELISAs, SDS-PAGE gels, and western blot assays for drug impurity screening. Flow cytometric analysis of platelet activation. Performed skills include: experimental design, stability studies, spike and recovery, dilution linearity, ULOQ, LLOQ, plate uniformity, cross reactivity, antibody biotinylation, data calculations using, Softmax Pro, cell counts, antibody labeling, platelet isolation, cell fixation, endotoxin testing. Other activities: Write and follow standard operating procedures and validations of newly developed ELISA, SDS-PAGE gels, and Western blots methods, troubleshooting, and investigation of assay failures.

ALPCO | Feb 2014 - Oct 2015

R&D Associate II

Immuno-assay development, validation, and documentation of ELISA kits within the diabetes and obesity team. Performed skills include: Antibody titrations, spike and recovery, linearity, ULOQ, LLOQ, plate washing, plate coating, data calculations using XLfit, Softmax Pro, and Gen5

Abazyme LLC | May 2013 - Feb 2014

Research Associate

Generation of recombinant monoclonal antibodies. Gene cloning of heavy and light chain antibodies in E.coli, purify plasmids, and transfected into HEK 293 cells and immuno-detection. Performed skills include: Generation of cDNA from mRNA extracts, RT-PCR, ELISA assays, colony PCR, restriction digests, bacterial mini-preps, gel electrophoresis, gene cloning, DNA isolation, passage of 293e cells, endotoxin removal, PCR, OD readings, electro and chemical transformation of E. coli cells.

Center of Biological Sciences: Hematology Research Lab | May 2012 - Sep 2012

Research Intern

Gene cloning of nuclear envelope protein cDNAs into mammalian expression vectors. Learned skills included: Restriction digests and restriction enzyme site mapping, DNA gel electrophoresis, Transfection and manipulations of mammalian cell cultures (HEK 293 fibroblasts and myeloblastic HL-60 cells), Western blot analyses, autoclaving, and full inventory.

Center for Cellular Neurobiology and Neurodegeneration Research | Jan 2012 - May 2012

Research Assistant

Electrode stimulation of primary cortical cells using Multi-electrode Array plates (MEA) in order to determine neural activity and plasticity of mouse embryonic neural cells. Learned skills included: MEA plate preparations, coating, and stimulations, culturing of NB2A and primary cortical cells, statistical data calculations using Microsoft Excel

Avid Technology | Oct 2009 - May 2011

Technical Support Engineer I

Technical Support Engineer for Media Composer Editor ACSR Certified for Avid Editing Systems ACSR Certified for Avid Unity MediaNetwork Onsite visits, troubleshooting, product assembly, CPU maintenance and customer care.

Lowell Telecommunications Corporation | Jun 2009 - Sep 2009

Intern - Broadcast Audio/Teacher/FCP Editor

Taught Girls Inc. media classes about how to use digital cameras, how to edit them in After Effects, how to use video cameras, and how to edit into film. Also, did a little Final Cut Pro editing for the broadcast of shows. Mixed the audio for live broadcasts at the station as well.

Bose Corporation | Nov 2008 - Jan 2009

Technical Support/ Customer Service

N/A

My Education

Take a look at my formal education

Master's degree, Bioinformatics: Data AnalyticsNortheastern University | 2024
Certificate, Python for Data ScienceUniversity of Chicago | 2021
Master of Science (M.S.), BiotechnologyUniversity of Massachusetts at Lowell | 2014
Bachelor of Science (B.S.), BiologyUniversity of Massachusetts at Lowell | 2012
B.M., Sound Recording TechnologyUniversity of Massachusetts Lowell | 2008

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