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Differential Expression Analysis with limma in R

高级技能水平
更新时间 2024年8月
Learn to use the Bioconductor package limma for differential gene expression analysis.
免费开始课程
RProbability & Statistics
4小时
15 视频
47 道练习
3,900 XP
8,122
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课程描述

Functional genomic technologies like microarrays, sequencing, and mass spectrometry enable scientists to gather unbiased measurements of gene expression levels on a genome-wide scale. Whether you are generating your own data or want to explore the large number of publicly available data sets, you will first need to learn how to analyze these types of experiments. In this course, you will be taught how to use the versatile R/Bioconductor package limma to perform a differential expression analysis on the most common experimental designs. Furthermore, you will learn how to pre-process the data, identify and correct for batch effects, visually assess the results, and perform enrichment testing. After completing this course, you will have general analysis strategies for gaining insight from any functional genomics study.

先决条件

Introduction to Statistics in R
1

Differential Expression Analysis

To begin, you'll review the goals of differential expression analysis, manage gene expression data using R and Bioconductor, and run your first differential expression analysis with limma.
开始章节
2

Flexible Models for Common Study Designs

In this chapter, you'll learn how to construct linear models to test for differential expression for common experimental designs.
开始章节
3

Pre- and post-processing

4

Case Study: Effect of Doxorubicin Treatment

In this final chapter, you'll use your new skills to perform an end-to-end differential expression analysis of a study that uses a factorial design to assess the impact of the cancer drug doxorubicin on the hearts of mice with different genetic backgrounds.
开始章节
Differential Expression Analysis with limma in R
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